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|metadata.artigo.dc.title:||Cupriavidus necator isolates are able to fix nitrogen in symbiosis with different legume species|
|metadata.artigo.dc.creator:||Silva, Krisle da|
Florentino, Ligiane Aparecida
Silva, Karina Barroso da
Brandt, Evie de
Moreira, Fatima Maria de Souza
|metadata.artigo.dc.subject:||Biological nitrogen fixation|
Fixação biológica de nitrogênio
|metadata.artigo.dc.identifier.citation:||SILVA, K. da et al. Cupriavidus necator isolates are able to fix nitrogen in symbiosis with different legume species. Systematic and Applied Microbiology, Stuttgart, v. 35, n. 3, p. 175-182, May 2012.|
|metadata.artigo.dc.description.abstract:||The aim of the present study was to identify a collection of 35 Cupriavidus isolates at the species level and to examine their capacity to nodulate and fix N2. These isolates were previously obtained from the root nodules of two promiscuous trap species, Phaseolus vulgaris and Leucaena leucocephala, inoculated with soil samples collected near Sesbania virgata plants growing in Minas Gerais (Brazil) pastures. Phenotypic and genotypic methods applied for this study were SDS-PAGE of whole-cell proteins, and 16S rRNA and gyrB gene sequencing. To confirm the ability to nodulate and fix N2, the presence of the nodC and nifH genes was also determined, and an experiment was carried out with two representative isolates in order to authenticate them as legume nodule symbionts. All 35 isolates belonged to the betaproteobacterium Cupriavidus necator, they possessed the nodC and nifH genes, and two representative isolates were able to nodulate five different promiscuous legume species: Mimosa caesalpiniaefolia, L. leucocephala, Macroptilium atropurpureum, P. vulgaris and Vigna unguiculata. This is the first study to demonstrate that C. necator can nodulate legume species.|
|Appears in Collections:||DCS - Artigos publicados em periódicos|
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