Análise fisiológica de embriões de Inga vera Willd. Subsp. affinis (DC.) T. D. Pennington armazenados em meio osmótico

Abstract

Among the peculiarities of seeds, the recalcitrance can be understood as a characteristic that makes the seed can not be stored and can not lose its water content without loss of viability. This phenomenon, which has not yet been studied mainly in forest seeds due to the large number of species and the different shing ways of reducing recalcitrance or at least prolonging the storage of these seeds. In addition, it is known that desiccation tolerance is controlled by several genes, which may or may notlevels of recalcitrance among them, deserves to be deepened and arouses interest for studies that aim at establi be expressed, in different parts of the plant. In this way, the objective of this work was to store Inga vera seeds, which have a recalcitrant behavior, in three different conditions, being cold chamber and polyethylene glycol in potentials -1.6 MPa and -2.4 MPa. In addition, the pollen grains of the species were counted, classified for desiccation tolerance and evaluated for the presence of pollenkit and starch. The osmotic storage controls the mobilization of water inside the seeds, so that their metabolism is reduced and the consumption of their reserves decelerated. The study was conducted at the Forest Seeds Laboratory located at Federal University of Lavras – UFLA, with Inga vera embryos collected in Lavras - MG and RibeirãoVermelho - MG. It was possible to conclude that Inga vera embryos can be successfully stored in polyethylene glycol solution at -1.6 MPa potential for up to 140 days without any decrease in their germinative percentage. The Inga vera seeds were classified as amylaceous, with results consistent between reduction of germination and reduction in the contents of reserves. The pollen grains of the species were classified as orthodox and without pollenkit. Thus, it is observed that the species Inga vera presents genes of desiccation tolerance, being these expressed in the pollen grains, but not in the seeds, being able to be related to the time of dispersion of these structures, suggesting that further studies should be carried out in order to understand the stimuli that may induce the seeds expressing such genes and seed can be dried and stored for even longer periods.