Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/29222
Título: GFP-fluorescent protein on the study of blister spot in coffee plants
Título(s) alternativo(s): A proteína fluorescente GFP no estudo da mancha manteigosa em plântulas de café
Palavras-chave: Cafeeiro - Mancha manteigosa
Colletotrichum gloeosporioides
Coffee plants - Blister spot
Data do documento: Abr-2015
Editor: Grupo Paulista de Fitopatologia
Citação: ARMESTO, C. et al. GFP-fluorescent protein on the study of blister spot in coffee plants. Summa Phytopathologica, Botucatu, v. 41, n. 2, p. 89-93, Apr./June 2015.
Resumo: In Brazil, Colletotrichum gloeosporioides is associated with a complex of symptoms in coffee culture. Although this pathogen had its pathogenesis observed and identified, its importance has still been questioned due to its several endophytic forms, raising doubts as to the real importance of the pathosystem. The aim of this study was to demonstrate, by using an isolate transformed with the gene gfp, the infection and colonization capability of C. gloeosporioides in coffee seedlings. After the fourth day of inoculation, manifestation of symptoms as punctual necrosis could be observed, which progressed during the evaluation period, culminating in the death of seedlings. Epifluorescence microscopy confirmed the presence of the pathogen in the seedlings, as well as the visualization of internal colonization of tissues, acervulus formation and conidium production, confirming that it was responsible for the observed symptoms.
URI: http://repositorio.ufla.br/jspui/handle/1/29222
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