Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/30146
Title: Optimization of jenipapo in vitro seed germination process
Other Titles: Otimização do processo de germinação in vitro de sementes de jenipapo
Keywords: Genipa americana L.
In vitro culture
Ligth quality
Rubiaceae
Stratification in the dark
Cultivo in vitro
Qualidade de luz
Estratificação no escuro
Issue Date: Dec-2016
Publisher: Editora da Universidade Federal de Lavras
Citation: SOUZA, R. R. de et al. Optimization of jenipapo in vitro seed germination process. Ciência e Agrotecnologia, Lavras, v. 40, n. 6, p. 658-664, Nov./Dec. 2016.
Abstract: The in vitro seed germination is an effective alternative for quickly obtaining explants with sanitary quality. However, jenipapo seeds present slow and uneven germination. Therefore, internal and external factors to seed which directly interfere in the process, they must be identified, in order to adapt better techniques to obtain seedlings. In this sense, this work aimed to optimize the in vitro germination of Genipa americana L. seeds by evaluating different factors (light quality, GA3 treatment, pre-soaking in distilled water, growing media and stratification in the dark). It was found that the seed germination of G. americana was indifferent to light, however, the best results were obtained under conditions of continuous darkness; There was no effect of the application of exogenous GA3; The pre-soaking in distilled water for 48 h contributes to obtaining better germination rates; And the reduction in MS medium salts, and laminating the pretreatment in the dark maximizes the germination potential of seeds.Therefore, the optimal conditions for in vitro germination of G. americana L. seeds requires pre-soaking in distilled water for 48 hours and inoculation into culture media consisting of 1/2 MS + 15 g L-1 sucrose, with stratification in the dark for 16 days, followed by the transfer to growth chambers with lighting provided by white fluorescent lamps.
URI: http://repositorio.ufla.br/jspui/handle/1/30146
Appears in Collections:DBI - Artigos publicados em periódicos

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