Protocolos de extração de RNA e atividade enzimática em sementes de pimenta (Capsicum chinense)

Abstract

In this work were tested four methods of ARN extraction (method CTAB, CTAB, hot borate, Pure Link Reagent and Trizol Reagent), in four different development stages of habanero pepper seeds (Capsicum chinense), with the objective to determine which method is more efficient to obtain higher quality and quantity of ARN. Beyond the ARN extraction, seeds were also submitted to enzymatic analysis of esterase, superoxide dismutase, α-amylase, β-amylase and endo-β-mannanase. The Pure Link Reagent method was the one, which presented the purest and without degradation RNAs in pepper seeds in the development stages tested, which may be used for the analysis of gene expression.