Please use this identifier to cite or link to this item:
metadata.artigo.dc.title: Tolerance to and accumulation of cadmium, copper, and zinc by Cupriavidus necator
metadata.artigo.dc.creator: Vicentin, Rayssa Pereira
Santos, Jessé Valentim dos
Labory, Cláudia Regina Gontijo
Costa, Amanda Monique da
Moreira, Fatima Maria de Souza
Alves, Eduardo
metadata.artigo.dc.subject: Heavy metals
Electron microscopy
Diazotrophic bacteria
Tolerance mechanisms
Metais pesados
Microscopia eletrônica
Bactérias diazotróficas
Mecanismos de tolerância
metadata.artigo.dc.publisher: Sociedade Brasileira de Ciência do Solo 2018
metadata.artigo.dc.identifier.citation: VICENTIN, R. P. et al. Tolerance to and accumulation of cadmium, copper, and zinc by Cupriavidus necator. Revista Brasileira de Ciência do Solo, Viçosa, MG, v. 42, p. 1-12, 2018. DOI: 10.1590/18069657rbcs20170080.
metadata.artigo.dc.description.abstract: Preliminary results of in vitro experiments with multicontaminated soils and solid media indicated that nodulating diazotrophic bacteria of the genus Cupriavidus are promising for the remediation of contaminated environments due to their symbiosis with legumes and metal tolerance. Thus, strains of Cupriavidus spp. (LMG 19424T, UFLA 01-659, UFLA 01-663, and UFLA 02-71) were tested for their ability to tolerate and bioaccumulate cadmium (Cd), copper (Cu), and zinc (Zn) in Luria-Bertani broth. Changes in the growth pattern of Cupriavidus strains in the presence or absence of heavy metals were analyzed by scanning electron microscopy and metal allocation by transmission electron microscopy, to clarify the mechanisms of bioremediation. Highest tolerance was detected for strain UFLA 01-659 (minimum inhibitory concentration of 5, 4.95, and 14.66 mmol L−1 of Cd, Cu, and Zn, respectively). Among the removal rates of the metals tested (9.0, 4.6, and 3.2 mg L−1 of Cd, Cu, and Zn, respectively), the bacterial activity was clearly highest for Cd. The efficiency of strain UFLA 01-659 in removing the heavy metals is associated with its high biomass production and/or higher contents of heavy metals adsorbed and absorbed in the biomass. In response to the presence of heavy metals in the liquid culture medium, the bacteria produced exopolysaccharides and small and aggregated cells. However, these responses varied according to the strains and heavy metals. Regarding allocation, all heavy metals were adsorbed on the cell wall and membrane, whereas complexation was observed intracellularly and only for Cu and Zn. These results indicate the possibility of using C. necator UFLA 01-659 for remediation in areas with very high Cd, Cu, and Zn contents.
metadata.artigo.dc.language: en_US
Appears in Collections:DFP - Artigos publicados em periódicos

Files in This Item:
File Description SizeFormat 
ARTIGO_Tolerance to and accumulation of cadmium, copper, and zinc by Cupriavidus necator.pdf873,48 kBAdobe PDFView/Open

This item is licensed under a Creative Commons License Creative Commons