Conservação de Genipa americana: técnicas de encapsulamento e crescimento lento

Abstract

Nodal segments of Genipa americana were stored using encapsulation and slow growth techniques. The influence of the 3% and 4% sodium alginate matrix, the effect of a pretreatment with 0, 0,25 and 0,50 M sucrose solutions, as well the effect of 8ºC and 15ºC temperature during storege for 30 days. It was evaluated the percentage of rupture of these capsules after 50 days of inoculation. For the slow growth, different sucrose concentrations (30, 45 and 60 g L -1 ) and temperature 8ºC and 15ºC after storege for 90 and 180 days were evaluated. It was evaluated the percentage of survival and regeneration, shoot lenght, shoots and leaves number. Only the encapsulating units stored at 15 °C survived. At this temperature, the capsule rupture was better in pretreatment with 0 M sucrose. For the slow growth, the temperature of 8ºC also did not allow the survival of the explants. Thus, after 180 days the best results occurred at the temperature of 15 °C with the addition of 60 g L -1 of sucrose to the culture medium. In this way, it can be concluded that it is possible to make the storage of the species using these techniques.