Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/36009
Title: Eficiência no uso do fósforo, parâmetros microbiológicos do solo e identificação e caracterização de genes da família das fosfatases ácidas em cafeeiros
Other Titles: Efficiency in the use of phosphorus, microbiological parameters of the soil and identification and characterization of acid phosphatases family genes in coffee
Authors: Carvalho, Gladyston Rodrigues
Botelho, Cesar Elias
Carneiro, Marco Aurélio Carbone
Silva, Douglas Ramos Guelfi
Cunha, Rodrigo Luz da
Oliveira, Antonio Carlos Baião de
Keywords: Coffea arabica
Cultivares
Nutrição mineral
Biomassa microbiana
Micorriza
Cultivars
Mineral nutrition
Microbial biomass
Mycorrhiza
Issue Date: 7-Aug-2019
Publisher: Universidade Federal de Lavras
Citation: VILELA, D. J. M. Eficiência no uso do fósforo, parâmetros microbiológicos do solo e identificação e caracterização de genes da família das fosfatases ácidas em cafeeiros. 2019. 116 p. Tese (Doutorado em Agronomia/Fitotecnia)–Universidade Federal de Lavras, Lavras, 2019.
Abstract: This work aimed to evaluate the nutritional efficiency regarding phosphorus (P), microbiological parameters of the soil and to identify and characterize genes of the family of acid phosphatases in coffee trees. The experiment was conducted under greenhouse conditions at the Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG), located in Lavras, state of Minas Gerais, Brazil. In Chapter 2, the experimental design followed a randomized complete block design, in a 10x2 factorial scheme (10 arabica coffee genotypes and two doses of phosphate fertilization), with four replications. In Chapter 3, the experimental design followed a completely randomized design, in a 2x2 factorial scheme (2 cultivars of arabica coffee and two doses of phosphate fertilization), with three replications. Each experimental plot consisted of one pot with ten liters of soil, with one plant. The soil used in the study was collected from the subsurface layer and did not receive any type of microbiological inoculation. Fertilization was carried out using nutrient solutions. The experiment lasted eight months in the greenhouse. Thereafter, the soil and plant material samples from each plot were collected for analysis. Agronomic, physiological, chemical, microbiological and molecular variables were analyzed. The cultivars Catiguá MG2, MGS Ametista and Sarchimor MG 8840 are neither efficient nor responsive to phosphate fertilization. Progeny H 6-47-10 pl. 3 and the cultivar Catuaí Vermelho IAC 144 are not efficient but are responsive to phosphate fertilization. The cultivars Paraíso MG H 419-1, Topázio MG 1190 and MGS Paraíso 2 are efficient but are not responsive to phosphate fertilization. The cultivars Bourbon Amarelo IAC J10 and MGS Aranãs are efficient and responsive to phosphate fertilization. Phosphate fertilization has a positive influence on the microbial biomass carbon and negatively on mycorrhizal spores density. Genotypes of arabica coffee respond positively both with phosphate fertilization (MGS Ametista) and without phosphate fertilization (MGS Paraíso 2) in acid phosphatase activity. A total of 29 genes of the Purple Acid Phosphatase (PAP) family in the genome of Coffea canephora were identified. In the cultivar MGS Paraíso 2 some PAPs were more expressed under P deprivation (CarPAP3, CarPAP11 and CarPAP18) and others were indifferent to P (CarPAP12). The gene expression of CarPAP18 and the acid phosphatase activity were highly significant in the MGS Paraíso 2 cultivar under P deprivation.
URI: http://repositorio.ufla.br/jspui/handle/1/36009
Appears in Collections:Agronomia/Fitotecnia - Doutorado (Teses)



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