Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/36381
metadata.artigo.dc.title: Addition of insulin-like growth factor I to the maturation medium of bovine oocytes subjected to heat shock: effects on the production of reactive oxygen species, mitochondrial activity and oocyte competence
metadata.artigo.dc.creator: Ascari, I. J.
Alves, N. G.
Jasmin, J.
Lima, R. R.
Quintão, C. C. R.
Oberlender, G.
Moraes, E. A.
Camargo, L. S. A.
metadata.artigo.dc.subject: Thermoprotection
Heat stress
DNA fragmentation
Mitochondrial membrane potential
ROS production
Termoproteção
Estresse por calor
Fragmentação de DNA
Potencial de membrana mitocondrial
Produção de ROS
metadata.artigo.dc.publisher: Elsevier
metadata.artigo.dc.date.issued: Jul-2017
metadata.artigo.dc.identifier.citation: ASCARI, I. J. et al. Addition of insulin-like growth factor I to the maturation medium of bovine oocytes subjected to heat shock: effects on the production of reactive oxygen species, mitochondrial activity and oocyte competence. Domestic Animal Endocrinology, Stoneham, v. 60, p. 50-60, July 2017.
metadata.artigo.dc.description.abstract: This study was performed to investigate the effects of insulin-like growth factor-I (IGF-I) addition to in vitro maturation (IVM) medium on apoptosis, mitochondrial membrane potential, ROS production, and developmental competence of bovine oocytes subjected to heat shock. Two temperatures (conventional: 24 h at 38.5°C, or heat shock: 12 h at 41°C followed by 12 h at 38.5°C) and 3 IGF-I concentrations (0, 25, and 100 ng/mL) were tested during IVM. The oocytes were then fertilized in vitro, and the presumptive zygotes were cultured until reaching the blastocyst stage. There was no interaction between temperature and IGF-I concentration for any variable evaluated (P > 0.05). The addition of IGF-I did not alter the proportion of nuclear maturation, TUNEL-positive oocytes and caspase-3 activity, or blastocyst proportion on Days 7 and 8 post-fertilization. Furthermore, the total number of cells and the number of cells in the inner cell mass (ICM) in the blastocyst were not altered (P > 0.05). However, IGF-I increased (P < 0.05) the mitochondrial membrane potential and the production of ROS in oocytes and decreased (P < 0.05) the proportion of apoptotic cells in the ICM in blastocysts. Heat shock increased (P < 0.05) the proportion of TUNEL-positive oocytes and ROS production and reduced (P < 0.05) the mitochondrial membrane potential. Moreover, heat shock increased (P < 0.05) the apoptosis proportion in the ICM cells. In conclusion, supplementing IVM medium with IGF-I may increase the mitochondrial membrane potential and ROS production in oocytes and decrease apoptosis in the ICM in blastocysts. Heat shock for 12 h compromised oocyte developmental competence and increased apoptosis within the ICM cells of the blastocysts.
metadata.artigo.dc.identifier.uri: https://www.sciencedirect.com/science/article/pii/S0739724017300589?via%3Dihub#!
http://repositorio.ufla.br/jspui/handle/1/36381
metadata.artigo.dc.language: en_US
Appears in Collections:DES - Artigos publicados em periódicos

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