Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/37287
Title: Caracterização morfológica de calos de Eucalyptus urophylla S. T. Blake 215 sob concentrações de boro e cálcio
Other Titles: Morphological characterization of calli of Eucalyptus urophylla S. T. Blake subjected to concentrations of boron and calcium
Keywords: Meio de cultura
Nutrição mineral
Cultivo in vitro
Cultura de tecidos
Rizogênese
Culture medium
Mineral nutrition
In vitro cultivation
Tissue culture
Rhizogenesis
Issue Date: 2011
Publisher: Universidade Federal de Lavras
Citation: TREVIZAM, R. et al. Caracterização morfológica de calos de Eucalyptus urophylla S. T. Blake 215 sob concentrações de boro e cálcio. Cerne, Lavras, v. 17, n. 2, p. 215-222, abr./jun. 2011.
Abstract: This study aimed to evaluate the effect of B and Ca concentrations on morphological responses and size of calli in Eucalyptus urophylla. Calli were obtained from the mid portion of hypocotyls removed from in vitro germinated seedlings. The callus structures were cultivated for 21 and 31 days, being kept in a modifi ed N7 medium with concentrations of B (0; 25; 50; 100 and 200 μM H3 BO3 ) and Ca (0; 3.75; 7.5; 11.25 and 15 mM CaCl2 .2H2 O). Size of calli, presence of roots, friability, viscosity, presence of globular structures on the surface and presence of pigments were analyzed. Overall, the shorter culture period (21 days) allowed better development of callus structures. The combination of 50 μM H3 BO3 with 7.5 mM CaCl2 .2H2 O at 21 days, and 100 μM H3 BO3 with 1.13 mM CaCl2 .2H2 O at 31 days, provided best results. Rhizogenesis responded differently according to B and Ca concentrations. Simultaneous omission of B and Ca inhibited the rhizogenesis, causing disruption of the callus, favored formation of globular and friable structures, with presence of anthocyanin. High concentrations of calcium promoted root induction in calli.
URI: http://repositorio.ufla.br/jspui/handle/1/37287
Appears in Collections:DCF - Artigos publicados em periódicos



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