Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/37621
Title: Tumor growth reduction is regulated at the gene level in walker 256 tumor-bearing rats supplemented with fish oil rich in EPA and DHA
Keywords: Cancer
Fish oil
Gene expression
Issue Date: 2013
Publisher: Associação Brasileira de Divulgação Científica
Citation: BORGHETTI, G. et al. Tumor growth reduction is regulated at the gene level in walker 256 tumor-bearing rats supplemented with fish oil rich in EPA and DHA. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 46, n. 8, p. 696-699, 2013.
Abstract: We investigated the effect of fish oil (FO) supplementation on tumor growth, cyclooxygenase 2 (COX-2), peroxisome proliferator-activated receptor gamma (PPARc), and RelA gene and protein expression in Walker 256 tumor-bearing rats. Male Wistar rats (70 days old) were fed with regular chow (group W) or chow supplemented with 1 g/kg body weight FO daily (group WFO) until they reached 100 days of age. Both groups were then inoculated with a suspension of Walker 256 ascitic tumor cells (36107 cells/mL). After 14 days the rats were killed, total RNA was isolated from the tumor tissue, and relative mRNA expression was measured using the 2-DDCT method. FO significantly decreased tumor growth (W=13.18±1.58 vs WFO=5.40±0.88 g, P,0.05). FO supplementation also resulted in a significant decrease in COX-2 (W=100.1±1.62 vs WFO=59.39±5.53, P,0.001) and PPARc (W=100.4±1.04 vs WFO=88.22±1.46, P,0.05) protein expression. Relative mRNA expression was W=1.06±0.022 vs WFO=0.31±0.04 (P,0.001) for COX-2, W=1.08±0.02 vs WFO=0.52±0.08 (P,0.001) for PPARc, and W=1.04±0.02 vs WFO=0.82±0.04 (P,0.05) for RelA. FO reduced tumor growth by attenuating inflammatory gene expression associated with carcinogenesis.
URI: http://repositorio.ufla.br/jspui/handle/1/37621
Appears in Collections:DNU - Artigos publicados em periódicos



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