Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/37863
Title: Genetic variability among cassava accessions based on SSR markers
Other Titles: Variabilidade genética entre acessos de mandioca com base em marcadores SSR
Keywords: Manihot esculenta
Microssatélites
Microsatellites
Issue Date: 2011
Publisher: Crop Breeding and Applied Biotechnology
Citation: RIBEIRO, M. de N. O.; CARVALHO, S. P. de; SANTOS, J. B. dos; ANTONIO, R. P. Genetic variability among cassava accessions based on SSR markers. Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 11, n. 3, p. 263-269, 2011.
Abstract: The aim of this study was to characterize and estimate the genetic similarity among 93 cassava accessions. The DNA amplification was performed with 14 microsatellite primers. The amplification products were separated by a polyacrylamide gel electrophoresis, showing a polymorphism formation, through which the accessions were discriminated against. The genetic similarity among accessions of cassava was estimated by the Dice coefficient. Cluster analysis was carried out using the UPGMA method. The polymorphic primers amplified a total of 26 alleles with 2-4 alleles per loci. The genetic similarity ranged from 0.16 to 0.96. The average values for observed and expected heterozygosity were 0.18 and 0.46, respectively. Twenty genetic similarity clusters were determined, demonstrating diversity among accessions, suggesting the possibility of heterotic hybrid generation.
URI: http://repositorio.ufla.br/jspui/handle/1/37863
Appears in Collections:DBI - Artigos publicados em periódicos

Files in This Item:
File Description SizeFormat 
ARTIGO_Genetic variability among cassava accessions based on SSR markers.pdf756,71 kBAdobe PDFView/Open


This item is licensed under a Creative Commons License Creative Commons