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Title: | Development of molecular markers based on retrotransposons for the analysis of genetic variability in Moniliophthora perniciosa |
Keywords: | Retrotransposon Moniliophthora perniciosa Genetic variability Interretrotransposon amplified polymorphism (IRAP) Retrotransposon-microsatellite amplified polymorphism (REMAP) |
Issue Date: | 2012 |
Publisher: | Springer |
Citation: | SANTANA, M. F. et al. Development of molecular markers based on retrotransposons for the analysis of genetic variability in Moniliophthora perniciosa. European Journal of Plant Pathology, [S.l.], v. 134, p. 497-507, 2012. |
Abstract: | Moniliophthora perniciosa is a fungus that causes witches’ broom disease (WBD) in the cacao tree (Theobroma cacao). The M. perniciosa genome contains different transposable elements; this prompted an evaluation of the use of its retrotransposons as molecular markers for population studies. The inter-retrotransposon amplified polymorphism (IRAP) and retrotransposon-microsatellite amplified polymorphism (REMAP) techniques were used to study the variability of 70 M. perniciosa isolates from different geographic origins and biotypes. A total of 43 loci was amplified. Cluster analysis of different geographical regions of C biotype revealed two large groups in the state of Bahia, Brazil. Techniques using retrotransposon-based molecular markers showed advantages over previously used molecular techniques for the study of genetic variability in M. perniciosa. |
URI: | https://link.springer.com/article/10.1007/s10658-012-0031-4 http://repositorio.ufla.br/jspui/handle/1/38181 |
Appears in Collections: | DFP - Artigos publicados em periódicos |
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