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http://repositorio.ufla.br/jspui/handle/1/38369| Title: | Survival of sugarcane shoot tips after cryopreservation by droplet-vitrification |
| Other Titles: | Sobrevivência de ápices caulinares de cana-de-açúcar após criopreservação por "droplet-vitrification" |
| Keywords: | Saccharum In vitro conservation PVS2 Regeneration Plant vitrification solution 2 (PVS2) Conservação in vitro Regeneração |
| Issue Date: | Nov-2013 |
| Publisher: | Empresa Brasileira de Pesquisa Agropecuária (Embrapa), Secretaria de Pesquisa e Desenvolvimento (SPD) |
| Citation: | NOGUEIRA, G. F.; PASQUAL, M.; SCHERWINSKI-PEREIRA, J. E. Survival of sugarcane shoot tips after cryopreservation by droplet-vitrification. Pesquisa Agropecuária Brasileira, Brasília, v. 48, n. 11, p. 1524-1527, Nov. 2013. DOI: 10.1590/S0100-204X2013001100014. |
| Abstract: | The objective of this work was to evaluate the phytotoxicity of a plant vitrification solution (PVS2), and the survival of shoot tips of the sugarcane variety SP716949, after cryopreservation by droplet-vitrification. Shoot tips were precultured for 24 hours in MS medium containing 0.3 mol L-1 sucrose, and exposed to PVS2 for 0, 20 or 30 min. Shoot tips were then immersed in liquid nitrogen. Thawing was fast in concentrated sucrose solution (1.2 mol L-1). PVS2 is a nontoxic to shoot tips, which in turn are sensitive to liquid nitrogen. The best results occurred when shoot tips were maintained for up to 20 min in PVS2 solution, before freezing, with 20% survival. |
| URI: | http://repositorio.ufla.br/jspui/handle/1/38369 |
| Appears in Collections: | DAG - Artigos publicados em periódicos |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| ARTIGO_Survival of sugarcane shoot tips after cryopreservation by droplet-vitrification.pdf | 485,04 kB | Adobe PDF | View/Open |
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