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metadata.artigo.dc.title: Evaluation of hemagglutination activity of chitosan nanoparticles using human erythrocytes
metadata.artigo.dc.creator: Lima, Jefferson Muniz de
Sarmento, Ronaldo Rodrigues
Souza, Joelma Rodrigues de
Brayner, Fábio André
Feitosa, Ana Paula Sampaio
Padilha, Rafael
Alves, Luiz Carlos
Porto, Isaque Jerônimo
Batista, Roberta Ferreti Bonan Dantas
Oliveira, Juliano Elvis de
Medeiros, Eliton Souto de
Bonan, Paulo Rogério Ferreti
Castellano, Lúcio Roberto
metadata.artigo.dc.publisher: Hindawi 2015
metadata.artigo.dc.identifier.citation: LIMA, J. M. de et al. Evaluation of hemagglutination activity of chitosan nanoparticles using human erythrocytes. Journal of Nanoscience and Nanotechnology, [S.l.], v. 15, p. 5628-5636, 2015.
metadata.artigo.dc.description.abstract: Chitosan is a polysaccharide composed of randomly distributed chains of β-(1-4) D-glucosamine and N-acetyl-D-glucosamine. This compound is obtained by partial or total deacetylation of chitin in acidic solution. The chitosan-based hemostatic agents have been gaining much attention in the management of bleeding. The aim of this study was to evaluate in vitro hemagglutination activity of chitosan nanoparticles using human erythrocytes. The preparation of nanoparticles was achieved by ionotropic gelification technique followed by neutralization with NaOH 1 mol/L−1. The hemagglutination activity was performed on a solution of 2% erythrocytes (pH 7.4 on PBS) collected from five healthy volunteers. The hemolysis determination was made by spectrophotometric analysis. Chitosan nanoparticle solutions without NaOH addition changed the reddish colour of the wells into brown, suggesting an oxidative reaction of hemoglobin and possible cell lysis. All neutralized solutions of chitosan nanoparticles presented positive haemagglutination, without any change in reaction color. Chitosan nanoparticles presented hemolytic activity ranging from 186.20 to 223.12%, while neutralized solutions ranged from 2.56 to 72.54%, comparing to distilled water. Results highlight the need for development of new routes of synthesis of chitosan nanoparticles within human physiologic pH.
metadata.artigo.dc.language: en_US
Appears in Collections:DEG - Artigos publicados em periódicos

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