Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/38867
Title: Effect of DMSO on castor bean pollen grain viability
Keywords: Ricinus communis L.
Cryoprotectant solution
Acetic carmine
Issue Date: 30-Sep-2011
Publisher: International Society for Horticultural Science (ISHS)
Citation: VARGAS, D. P. et al. Effect of DMSO on castor bean pollen grain viability. Acta Horticulturae, [S.l.], v. 908, p. 93-96, Sept. 2011. Proceedings of the I International Symposium on Cryopreservation in Horticultural Species, Leuven, Belgium, 2009. DOI: 10.17660/ActaHortic.2011.908.8.
Abstract: Castor bean (Ricinus communis L.) is an oleaginous plant of high economic value in Brazil due to its use in the industrial (biodiesel production) and medicinal areas. The objective of this study was to evaluate the effect of the use of the cryoprotectant DMSO on the maintenance of pollen grain integrity under different storage temperatures. Pollen grains were extracted from anthers of male flowers and the viability was determined using the cytological stain 2% (w/v) acetic carmine and maintained in liquid nitrogen (-196°C), ultra freezer (-80°C) and freezer (-20°C) for 30 days with 10% DMSO as a cryoprotectant. Viability of 73.25 and 68.75% was obtained in pollen samples stored at -196 and -20°C, respectively. The cryopreservation in ultra-freezer (-80°C) showed a lower average of viable cells (42%), demonstrating the inefficiency of the storage in this temperature for the preservation of castor bean pollen grains.
URI: https://www.actahort.org/books/908/908_8.htm
http://repositorio.ufla.br/jspui/handle/1/38867
Appears in Collections:DBI - Artigos publicados em periódicos

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.