Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/38993
metadata.artigo.dc.title: Shoot-tip cryopreservation by droplet vitrification of Byrsonima intermedia A. Juss.: a woody tropical and medicinal plant species from Brazilian Cerrado
metadata.artigo.dc.creator: Silva, Luciano Coutinho
Paiva, Renato
Swennen, Rony
Andrè, Edwige
Panis, Bart
metadata.artigo.dc.subject: Pre-culture effect
Post-culture effect
Pre-growth effect
Sorbitol
Medicinal plant
Vitrification
metadata.artigo.dc.publisher: CryoLetters LLP
metadata.artigo.dc.date.issued: 2013
metadata.artigo.dc.identifier.citation: SILVA, L. C. et al. Shoot-tip cryopreservation by droplet vitrification of Byrsonima intermedia A. Juss.: a woody tropical and medicinal plant species from Brazilian Cerrado. Cryo-Letters, [S.l.], v. 34, n. 4, p. 338-348, July/Aug. 2013.
metadata.artigo.dc.description.abstract: Cryopreservation of plant species is poorly investigated in Brazil. The aim of this study was to cryopreserve Byrsonima intermedia shoot apical meristems through droplet vitrification. A culture medium for shoot-tips growth was established using the Woody Plant Medium supplemented with 2.22 µM 6-benzylaminopurine. Excised shoot-tips were subjected to pre-culture and/or post-culture treatments on Murashige and Skoog medium with 0.3 M sucrose for 24 h prior dehydration on PVS2 at 0°C for 15, 30 or 45 minutes prior to plunging in liquid nitrogen. The effect of 15 days of shoot pre-growth on a high osmotic medium (0.3 M sucrose or 0.21 M sorbitol + 0.09 M sucrose) prior to meristem excision and cryopreservation was also investigated. Pre-culturing shoot-tips on 0.3 M sucrose for 24 h prior to cryopreservation increased the regrowth level after thawing to 90%. Shoot-tips excised from shoots pre-grown on MS + 0.21 M sorbitol + 0.09 M sucrose for 15 days presented a satisfactory regrowth level (67%).
metadata.artigo.dc.identifier.uri: http://www.cryoletters.org/Abstracts/vol_34_4_2013.htm#338
http://repositorio.ufla.br/jspui/handle/1/38993
metadata.artigo.dc.language: en_US
Appears in Collections:DBI - Artigos publicados em periódicos

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