Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/40757
metadata.artigo.dc.title: Effect of the gastrointestinal environment on pH homeostasis of Lactobacillus plantarum and Lactobacillus brevis cells as measured by real-time fluorescence ratio-imaging microscopy
metadata.artigo.dc.creator: Ramos, Cíntia Lacerda
Thorsen, Line
Ryssel, Mia
Nielsen, Dennis S.
Siegumfeldt, Henrik
Schwan, Rosane Freitas
Jespersen, Lene
metadata.artigo.dc.subject: Lactobacillus brevis
Lactobacillus plantarum
In vitro gastro-intestinal model
pHi
FRIM
Probiotic
Intracellular pH
Fluorescence ratio imaging microscopy (FRIM)
metadata.artigo.dc.publisher: Elsevier
metadata.artigo.dc.date.issued: Apr-2014
metadata.artigo.dc.identifier.citation: RAMOS, C. L. et al. Effect of the gastrointestinal environment on pH homeostasis of Lactobacillus plantarum and Lactobacillus brevis cells as measured by real-time fluorescence ratio-imaging microscopy. Research in Microbiology, [S.l.], v. 165, n. 3, p. 215-225, Apr. 2014. DOI: 10.1016/j.resmic.2014.02.005.
metadata.artigo.dc.description.abstract: In the present work, an in vitro model of the gastrointestinal tract (GIT) was developed to obtain real-time observations of the pH homeostasis of single cells of probiotic Lactobacillus spp. strains as a measure of their physiological state. Changes in the intracellular pH (pHi) were determined using fluorescence ratio imaging microscopy (FRIM) for potential probiotic strains of Lactobacillus plantarum UFLA CH3 and Lactobacillus brevis UFLA FFC199. Heterogeneous populations were observed, with pHi values ranging from 6.5 to 7.5, 3.5 to 5.6 and 6.5 to 8.0 or higher during passage of saliva (pH 6.4), gastric (pH 3.5) and intestinal juices (pH 6.4), respectively. When nutrients were added to gastric juice, the isolate L. brevis significantly decreased its pHi closer to the extracellular pH (pHex) than in gastric juice without nutrients. This was not the case for L. plantarum. This study is the first to produce an in vitro GIT model enabling real-time monitoring of pH homeostasis of single cells in response to the wide range of pHex of the GIT. Furthermore, it was possible to observe the heterogeneous response of single cells. The technique can be used to determine the survival and physiological conditions of potential probiotics and other microorganisms during passage through the GIT.
metadata.artigo.dc.identifier.uri: https://www.sciencedirect.com/science/article/pii/S0923250814000278
http://repositorio.ufla.br/jspui/handle/1/40757
metadata.artigo.dc.language: en_US
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