Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/41225
Título : High throughput and comprehensive approach to develop multiepitope vaccine against minacious COVID-19
Autor: Ojha, Rupal
Gupta, Nidhi
Naik, Biswajit
Singh, Satyendra
Verma, Vijay Kumar
Prusty, Dhaneswar
Prajapati, Vijay Kumar
Palavras-chave: Adjuvant
Coronavirus
Multi-epitope
Vaccine
Immune response
Publicador: Elsevier
Data da publicação: Mai-2020
Referência: OJHA, R. et al. High throughput and comprehensive approach to develop multiepitope vaccine against minacious COVID-19. European Journal of Pharmaceutical Sciences, [S.l.], May 2020. No prelo.
Abstract: The ongoing enigmatic COVID-19 outbreak, first reported from Wuhan, China, on last day of the year 2019, which has spread to 213 countries, territories/areas till 28th April, 2020, threatens hundreds of thousands human souls. This devastating viral infection has stimulated the urgent development of viable vaccine against COVID-19 across the research institutes around the globe. The World Health Organization (WHO) has also confirmed that the recent pandemic is causing Public Health Emergency of International apprehension. Moreover, the earlier two pathogenic SARS-CoV and MERS-CoV and many others yet to be identified pose a universal menace. Here, in this piece of work, we have utilized an in silico structural biology and advanced immunoinformatic strategies to devise a multi-epitope subunit vaccine against ongoing COVID-19 infection. The engineered vaccine sequence is adjuvanted with ß-3 defensin and comprised of B-cell epitopes, HTL epitopes and CTL epitopes. This is very likely that the vaccine will be able to elicit the strong immune response. Further, specific binding of the engineered vaccine and immune cell receptor TLR3 was estimated by molecular interaction studies. Strong interaction in the binding groove as well as good docking scores affirmed the stringency of engineered vaccine. The interaction is stable with minimal deviation in root-mean square deviation and root-mean-square fluctuation was confirmed by the molecular dynamics simulation experiment. The immune-simulation by C-ImmSim server, which mimics the natural immune environment, yielded more potent immune response data of B-cells, Th cells, Tc cells and IgG for vaccine. The encouraging data obtained from the various in-silico works indicated this vaccine as an effective therapeutic against COVID-19.
URI: https://www.sciencedirect.com/science/article/pii/S0928098720301640
http://repositorio.ufla.br/jspui/handle/1/41225
Idioma: en_US
Aparece nas coleções:FCS - Artigos sobre Coronavirus Disease 2019 (COVID-19)

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