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metadata.artigo.dc.title: Production and identification of doubled haploids in tropical maize
metadata.artigo.dc.creator: Battistelli, G.M.
Von Pinho, R. G.
Justus, A.
Couto, E. G. O.
Balestre, M.
metadata.artigo.dc.subject: Haploidy inducer
Chromosome duplication
SSR markers
Flow cytometry
Doubled haploid lines
Hybrid maize
Indutor de haploidia
Duplicação de cromossomos
Citometria de fluxo
Linhagens haplóides duplicadas
Milho híbrido
Simple Sequence Repeat (SSR)
metadata.artigo.dc.publisher: Fundação de Pesquisas Científicas de Ribeirão Preto - FUNPEC-RP Oct-2013
metadata.artigo.dc.identifier.citation: BATTISTELLI, G. M. et al. Production and identification of doubled haploids in tropical maize. Genetics and Molecular Research, Ribeirão Preto, v. 12, n. 4, p. 4230-4242, 2013. DOI: 10.4238/2013.October.7.9.
metadata.artigo.dc.description.abstract: The production of maize doubled haploid (DH) lines is a technique commonly used by private companies, but not by Brazilian public institutions. Research on this technique is essential to develop and improve the production of DH lines grown under tropical conditions. We assessed the ability of a gynogenetic haploid inducer system to induce haploids in a tropical environment, assessed the induction rate of haploids identified using the R-navajo morphological marker, checked for interference from the generation of hybrid donors on haploid induction, measured the ability of flow cytometry, and simple sequence repeat marker techniques to identify doubled haploids. Seeds from the inducer Krasnodar Embryo Marker Synthetic (KEMS) line were sown in Ponta Grossa, PR, and Cravinhos, SP, and the plants were crossed to produce six hybrids and their F2 generations. The seeds were separated according to the R-navajo morphological marker indicator of haploidy (purple endosperm and white embryo) and germinated in a controlled environment. Chromosomal duplication was performed in seedlings selected as putative haploids. We performed subsequent confirmation of ploidy and the success of duplication using flow cytometry and SSR marker techniques. We concluded that DH lines can be obtained from hybrids crossed with the inducer KEMS line. The generation of inbred hybrids did not affect the induction rate or chromosomal duplication in haploids. The use of flow cytometry and SSR markers was effective in verifying chromosomal duplication in haploids.
metadata.artigo.dc.language: en
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