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Title: Métodos de extração de DNA e seleção de primers de cpDNA para Ficus bonijesulapensis (Moraceae)
Other Titles: DNA extraction protocols and cpDNA primers to Ficus bonijesulapensis (Moraceae)
Keywords: DNA de cloroplasto
Protocolo CTAB
Protocolo Moog
Protocolo Bond
Regiões não codificantes
Extração de DNA
DNA chloroplastidial
CTAB protocol
Moog protocol
Bond protocol
Noncoding regions
DNA extraction
Issue Date: Dec-2010
Publisher: Universidade Federal Rural do Semi-Árido (UFERSA)
Citation: VIEIRA, F. de A. et al. Métodos de extração de DNA e seleção de primers de cpDNA para Ficus bonijesulapensis (Moraceae). Revista Caatinga, Mossoró, v. 23, n. 4, p. 69-74, out./dez. 2010.
Abstract: Phylogeography has become a powerful approach for elucidating contemporary geographical patterns of evolutionary subdivision within species and species complexes. The aim of this paper was to evalu-ate the extracted quality of DNA and amplification of noncoding chloroplast DNA for phylogeography studies in Ficus bonijesulapens. The comparative analysis of protocol DNA extraction was based in the CTAB method and protocol from Mogg and Bond. DNA samples were assessed for successful PCR amplification of universal primers for the amplification of noncoding regions of cpDNA. Amplified DNA was separated by electrophore-sis, stained with ethidium bromide and photographed under UV light. The protocol Moog e Bond produced the best DNA quality. This method will be used because it produces a high quality DNA in a short time and is less expensive. The primers HA, SG, BF, Q16, F32, FV, DT, CS and JA provided the strongest support for the in-ferred phylogeography, genetic diversity centers and for successful management in tree-conservation pro-grammes.
Appears in Collections:DCF - Artigos publicados em periódicos

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