Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/46160
Title: Molecular epidemiology and antimicrobial susceptibility of Staphylococcus aureus and Escherichia coli isolated from bovine mastitis
Other Titles: Epidemiologia molecular e susceptibilidade a antimicrobianos em Staphylococcus aureus e escherichia coli isoladas de mastite bovina
Authors: Guimarães, Alessandro de Sá
Dorneles, Elaine Maria Seles
Costa, Geraldo Márcio da
Cerqueira, Mônica Maria Oliveira Pinho
Coura, Fernanda Morcatti
Dorneles, Elaine Maria Seles
Keywords: Bovinos - Mastite
Zoonoses
Bovinos leiteiros - Doenças
Produção de leite
Multirresistência
Bovine - Mastitis
Zoonotic
Dairy cattle - Diseases
Issue Date: 24-Mar-2021
Publisher: Universidade Federal de Lavras
Citation: GONÇALVES, M. S. Molecular epidemiology and antimicrobial susceptibility of Staphylococcus aureus and Escherichia coli isolated from bovine mastites. 2021. 59 p. Dissertação (Mestrado em Ciências Veterinárias) – Universidade Federal de Lavras, Lavras, 2021.
Abstract: Brazil is the third largest milk producer in the world and infectious diseases, like bovine mastitis, are extremally important, since causes several economic losses to dairy industry. Besides that, some pathogens involved in mastitis pathogeny can also cause illness in humans, being a public health issue. Therefore, molecular studies that evaluate the dynamic of evolution of these pathogens, as well as distribution and risk factors, are critical to propose control measures and to monitor the interface between human and animal strains. In other to better understand the epidemiology and evolution of two important and zoonotic pathogens of bovine mastitis (Staphylococcus aureus and Escherichia coli) two studies were conducted with strains isolated from dairy farms in Minas Gerais state, Brazil. The first aimed to evaluate the genetic diversity of S. aureus isolated from dairy cows, using Multi-locus sequence typing (MLST), and the antimicrobial susceptibility profiles of these isolates. The second, in turn, aimed to compare the virulence profile and REP-PCR genotypes of E. coli isolated from subclinical mastitis, clinical mastitis isolates and dairy farm environment, and to access the virulence factors and genotypes potentially associated with the subclinical persistence into udder. Results showed a high diversity among bovine mastitis S. aureus and a great number of new STs were found. Proximity between S. aureus isolates from human and animal origin was also observed, as well as high resistance to penicillin and tetracyclines and isolates resistant to methicillin (MRSA). Regarding E. coli, it was observed that flagella seems to be a determinant virulence factor in subclinical and persistent infections by this pathogen in bovine mammary gland. Results of molecular typing by REP-PCR suggest that subclinical mastitis isolates are less genetically diverse than clinical mastitis and dairy farm environmental isolates, although it was not possible to determine a specific genotype associated with subclinical and persistent E. coli mastitis (MPEC).
URI: http://repositorio.ufla.br/jspui/handle/1/46160
Appears in Collections:Ciências Veterinárias - Mestrado (Dissertações)



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.