Panus lecomtei e Pleurotus pulmonarius: análise genômica e potencial enzimático dos macrobasidiomicetos aplicados a biomassas lignocelulósicas

Abstract

The application of biological pre-treatments using macrofungi has been a viable alternative to obtain fermentable sugars from lignocellulosic biomass due to the facility that these microorganisms naturally have to selectively biodegrade natural polymers. Thus, the application of these has been studied to increase the possibilities of using vegetable biomass adding value as biorefineries. Studies involving genomics in macrofungi have been used to understand the processes and genes involved in the biodegradation of lignocellulose. Another fact is linked to the preservation of the phenotypic characteristics of these fungi, which is still little explored for some species. Thus, in this work, the genome of the species Panus lecomtei CC40 and Pleurotus pulmonarius EF88 was assembled and annotated to better understand the genes and processes related to the biodegradation of lignocellulosic biomass. The sequencing was done with the platforms Illumina and PacBio. A hybrid assembly of 49 Mb of the genome of P. lecomtei CC40 was performed with 16,562 predicted genes and 71 MB in size for P. pulmonarius EF88 with 25,367 predicted genes. In both species, it was possible to identify families of CAZyme proteins, related to the biodegradation of cellulose, hemicellulose, and lignin polymers. Also, cytochrome P450 monooxygenases were noted, which are related to biodegradation of phenolic and xenobiotic compounds. Also, the cryopreservation of P. lecomtei CC40 was performed in this work to assess the viability of the production of laccase, an enzyme active in the deconstruction of plant cell walls. As a result, the viability of the cryopreserved strains was obtained, mainly when reactivated in BDA and Malt culture media. In addition, the cryopreserved strains dissipated the production of laccase in solid-state fermentation in palm oil pressing fiber, which shows that the cryopreservation process did not interfere with the enzyme activity.