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metadata.artigo.dc.title: Effectiveness of Core-Shell Nanofibers Incorporating Amphotericin B by Solution Blow Spinning Against Leishmania and Candida Species
metadata.artigo.dc.creator: Gonçalves, Ingrid Morgana Fernandes
Rocha, Ítalo Martins
Pires, Emanuene Galdino
Muniz, Isis de Araújo Ferreira
Maciel, Panmella Pereira
Lima, Jefferson Muniz de
Santos, Iêda Maria Garcia dos
Batista, Roberta Bonan Dantas
Medeiros, Eudes Leonnan Gomes de
Medeiros, Eliton Souto de
Oliveira, Juliano Elvis de
Goulart, Luiz Ricardo
Bonan, Paulo Rogério Ferreti
Castellano, Lúcio Roberto Cançado
metadata.artigo.dc.subject: Amphotericin B
Drug delivery systems
Anfotericina B
metadata.artigo.dc.publisher: Frontiers Oct-2020
metadata.artigo.dc.identifier.citation: GONÇALVES, I. M. F. et al. Effectiveness of Core-Shell Nanofibers Incorporating Amphotericin B by Solution Blow Spinning Against Leishmania and Candida Species. Frontiers in Bioengineering and Biotechnology, [S. I.], v. 8, Oct. 2020. DOI: 10.3389/fbioe.2020.571821.
metadata.artigo.dc.description.abstract: The aim of this study was to develop polymeric nanofibers for controlled administration of Amphotericin B (AmpB), using the solution centrifugation technique, characterizing its microstructural and physical properties, release rate, and activity against Leishmania and Candida species. The core-shell nanofibers incorporated with AmpB were synthesized by Solution Blow Spinning (SBS) and characterized by scanning electron microscopy (SEM), differential scanning calorimetry, X-Ray diffraction, and drug release assay. In vitro leishmanicidal and antifungal activity were also evaluated. Fibrous membranes with uniform morphology and smooth surfaces were produced. The intensity of the diffraction peaks becomes slightly more pronounced, assuming the increased crystallization in PLA/PEG at high AmpB loadings. Drug release occurred and the solutions with nanofibers to encourage greater incorporation of AmpB showed a higher concentration. In the results of the experiment with promastigotes, the wells treated with nanofibers containing concentrations of AmpB at 0.25, 0.5, and 1%, did not have any viable cells, similar to the positive control. Various concentrations of AmpB improved the inhibition of fungal growth. The delivery system based on PLA/PEG nanofibers was properly developed for AmpB, presenting a controlled release and a successful encapsulation, as well as antifungal and antileishmanial activity.
metadata.artigo.dc.language: en
Appears in Collections:DEG - Artigos publicados em periódicos

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