Insuflação uterina com ozônio em éguas com endometrite induzida por lipopolissacarídeo de Escherichia Coli

Abstract

This study aimed to evaluate whether applications of uterine insufflation with ozone in mares induced to endometritis applying lipopolysaccharide (LPS) from Escherichia coli (E. coli) can reduce the number of inflammatory cells in the uterus after treatment. Twenty mares were submitted to cytological tests, biopsy, culture, and blood count, randomly allocated in three treatments: Ozone Treatment1 (TO1), which consisted of applying the gas directly to the uterus, through insufflation for 6 minutes, at a concentration of 35 mg /mL, 0.25 L/mm of flow and 1.5 liters of gas for each animal; Ozone Treatment2 (TO2), which consisted of the same procedure as treatment 1, however, performed on two consecutive days; and Treatment 3 Negative control (TCN), in which the animals did not receive LPS, but only one application of ozone, as described in treatment 1. Two evaluations were performed, the first comparing the treatments and the second comparing the cytology within each of the treatments. treatments. In the first analysis (between treatments) the first 4 cytologies had similar values, however in cytology 5 the values of polymorphonuclear cells (PMNs) were different (P=0.01), being TO1 (0.21±2.7) and TO2 (10.6±2.9). When the analyzes were comparing the cytology performed in each treatment, the PMN values were different in treatment 1 (P = 0.01), in treatment 2 (P<0.001) and in TCN (P=0.002). Biopsy and culture samples were collected on D0 and D4, the histopathological analyzes were not different between the groups evaluated, therefore, the procedures performed did not cause any apparent damage to the endometrium. In relation to culture, TO1, 12.5% of the animals that had bacteria in culture 1 became negative in culture 2, in TO2 28.6% the results suggest that only one application of O3 was necessary to reduce the amount of PMNs in the cytological samples, being beneficial for the treatment of endometritis, and that two applications were more efficient in terms of reducing the amount of microorganisms in the culture.