Análise comparativa de perfis de expressão de C. arabica submetidos a estresses abióticos com dados de bibliotecas genômicas de New C.canephora e C. eugeniodes

Abstract

Gene expression analysis has been widely used as a method to study the complex signaling and metabolic pathways underlying cellular and developmental processes in biological organisms, including plants. An increasing number of studies of expression levels of various genes in plants are being carried out to understand the cellular and molecular mechanisms involved in plant development and growth, as well as in responses to biotic (pathogen infection) and abiotic (environmental) stresses. . High and low temperatures, drought, high salinity, inadequate light exposure are abiotic factors that affect plant growth and cause crop losses. In this way, understanding how plants perceive these environmental signals and how these are translated by their genetic machinery in order to minimize damage and adapt is of paramount importance. Many studies investigate the effects of singular and combined stresses and have enabled the elucidation of the complex molecular interaction that governs responses to plant stress. Plants activate both specific and non-specific responses to stresses caused by factors related to adverse climatic events, allowing greater efficiency in the response and conserving resources for growth. Signal specificity is achieved through the interaction between hormones of different metabolic pathways, such as ABA (absisic acid), AS (salicylic acid) and AJ (jasmonic acid). Transcription factors, heat shock, reactive oxygen species and small RNAs are also involved in the coordination of such responses. Emphasizing, transcription factors are one of the main responsible for the specificity of responses to stress. The manipulation of these provides one of the greatest opportunities to confer tolerance through genetic engineering, as they provide a series of downstream events (XU et al., 2011). These factors interact with elements of the promoter regions of stress-related genes, promoting an increase in the expression of several genes, thus allowing stress tolerance. (AGARWAL; JHAa, 2010). A family of genes known as C-repetitive binding factors (CBFs) or dehydration-responsive binding element factors (DREBs) are key components that implicate adaptation to drought, salinity and cold and regulate stress-responsive genes which modulate physiological adaptations. to plant abiotic stresses. The relationship of these factors makes up an important way of gene regulation and metabolic pathways dependent and not dependent on abscisic acid. The high expression of DREB genes causes the upregulation of genes regulated by cold (CORE) and genes responsive to osmotic stress, resulting in improved tolerance to abiotic stresses (CHEN et al., 2009). Thus, the aim of this work was to evaluate the use of different reference genomes (C.arabica, C.canephora and C. eugenioides) in the mapping of reads obtained by RNA-seq and to observe the effects in the comparison of the transcriptome of coffee plants when subjected to different abiotic stresses.