Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/57252
Title: Cross-reactivity of anti-human cytokine monoclonal antibodies used as a tool to identify novel immunological biomarkers in domestic ruminants
Keywords: Cytokines
Immunological biomarkers
Cross-reactivity
Citocinas
Biomarcadores imunológicos
Reatividade cruzada
Issue Date: 2015
Publisher: Fundação de Pesquisas Científicas de Ribeirão Preto
Citation: DORNELES, E. M. S. et al. Cross-reactivity of anti-human cytokine monoclonal antibodies used as a tool to identify novel immunological biomarkers in domestic ruminants. Genetics and Molecular Research, [S. l.], v. 14, n. 1, p. 940-951, 2015.
Abstract: Eleven commercially available PE-labeled anti-human (IL-1-α, IL-6, IL-8, TNF-α, IL-17A, IL-5, IL-10, IL-12 and IL-13) and anti-mouse (IL-10, TNF-α) cytokine monoclonal antibodies (mAbs) were tested for cross-reactivity with cattle, goat, and sheep cytokines. Cross-reactivity was assessed by comparative analysis with the standard reactivity of the target species. Our data demonstrated that anti-human IL-1-α, IL-6, IL-8, IL-17A and IL-10 mAbs cross-react with all ruminant species tested. Anti-human IL-5 mAb showed a strong cross-reactivity with cattle and goat IL-5, while anti-human TNF-α mAb showed a selective cross-reactivity with goat TNF-α. No cross-reactivity with the ruminant cytokines was observed for anti-human IL-12 and IL-13 mAbs or for the two anti-mouse cytokine mAbs tested. The present study demonstrated the cross-reactivity of various anti-human cytokine mAbs with cattle, sheep, and goat cytokines, increasing the range of immunological biomarkers for studies in veterinary medicine.
URI: https://www.geneticsmr.com/articles/crossreactivity-of-antihuman-cytokine-monoclonal-antibodies-used-as-a-tool-to-identify-novel-immunological-biomarkers-in.pdf
http://repositorio.ufla.br/jspui/handle/1/57252
Appears in Collections:DMV - Artigos publicados em periódicos

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