Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/57533
Title: Addition of IGF-I to storage-cooled boar semen and its effect on sperm quality
Keywords: Insulin-like growth factor I
Boar spermatozoa
Motility
Functional membrane integrity
Lipid peroxidation
Fructose uptake
Suíno - Espermatozoide
Sêmen - Resfriamento
Esperma - Qualidade
Issue Date: Dec-2011
Publisher: Elsevier
Citation: SILVA, D. M. et al. Addition of IGF-I to storage-cooled boar semen and its effect on sperm quality. Growth Hormone & IGF Research, [S. I.], v. 21, n. 6, p. 325-330, Dec. 2011.
Abstract: Objective To evaluate in vitro IGF-I treatment during warming of storage-cooled boar semen and its effect on seminal quality parameters and metabolism in spermatic cells. Design Semen samples (n = 7) warmed after stored at 15 °C for 24 or 72 h were divided into four equal parts. Different IGF-I concentrations (0, 50, 100 and 150 ng/mL) were added to the semen samples. The samples were incubated at 37 °C, and assessments were made after 0 and 120 min of incubation. Results For semen samples that were stored for 24 h, the addition of IGF-I had no effect (p > 0.05) on the total motility and intensity of movements by spermatic cells, osmotic resistance, live:dead cell ratio or total spermatic abnormalities. However, incubation with 150 ng/mL IGF-I did decrease glutathione peroxidase activity (p < 0.05) and reduce lipid peroxidation after 120 min of incubation. For semen samples stored for 72 h and incubated with IGF-I for 120 min, there was a linear relationship between the IGF-I concentration and the live:dead ratio (p < 0.05). There was a quadratic relationship between the IGF-I concentration and both the osmotic resistance (peak results at IGF-I = 62.4 ng/mL) and glutathione peroxidase activity (peak results at IGF-I = 77.8 ng/mL). There was no effect on lipid peroxidation (p > 0.05) after 120 min of incubation. Addition of IGF-I also decreased fructose utilization by spermatic cells regardless of semen storage time (p < 0.05). Conclusion This study suggests that IGF-I may be beneficial to semen stored for longer periods of time. Adding 150 ng/mL IGF-I improved the quality of semen stored for 24 h, and adding 78 ng/mL IGF-I improved the quality of semen stored for 72 h.
URI: https://www.sciencedirect.com/science/article/pii/S1096637411000980?via%3Dihub#!
http://repositorio.ufla.br/jspui/handle/1/57533
Appears in Collections:DMV - Artigos publicados em periódicos

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