Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/57776
Title: Effect of the addition of IGF-I and vitamin E to stored boar semen
Keywords: a-tocopherol
Antioxidant
Boar spermatozoa
Insulin-like growth factor-I
Lipid peroxidation
Suínos - Espermatozoide
Sêmen - Armazenamento
Issue Date: May-2013
Publisher: Cambridge University Press
Citation: MENDEZ, M. F. B. et al. Effect of the addition of IGF-I and vitamin E to stored boar semen. Animal, Cambridge, v. 7, n. 5, p. 793-798, May 2013.
Abstract: The objective of this study was to evaluate the addition of IGF-I to pig insemination doses stored at 15°C, in conjunction with the addition of different amounts of vitamin E (α-tocopherol). Semen samples (n = 12) from four boars were treated by the addition of different concentrations of vitamin E, ranging up to 400 μg/ml. Immediately after processing and after the doses had been stored at 15°C for 24 or 72 h, samples were warmed at 37°C and 30 ng/ml of IGF-I was added. The assessments were made after 10 and 120 min of IGF-I addition. There was a minor effect of the vitamin E added before cooling and IGF-I added after storage on sperm quality. The addition of 400 μg/ml of vitamin E to diluted semen reduced (P < 0.01) the malondialdehyde (MDA) production in boar semen stored at 15°C for 72 h, regardless of the addition of IGF-I as additive during a 120 min incubation period at 37°C. In these conditions, IGF-I also reduced (P < 0.05) the MDA production in semen samples without addition of vitamin E. IGF-I in the presence of vitamin E reduced (P = 0.03) the glucose intake in freshly diluted boar semen samples before cooling. It was concluded that the addition of 400 μg/ml of vitamin E reduces the MDA production in boar semen stored at 15°C for 72 h, regardless of the presence of IGF-I additive. The addition of IGF-I in doses stored for 72 h with vitamin E ensures higher sperm motility after 120 min of incubation at 37°C.
URI: https://www.cambridge.org/core/journals/animal/article/effect-of-the-addition-of-igfi-and-vitamin-e-to-stored-boar-semen/83A3D4711EA41A4F0CAA6002BBA31D49
http://repositorio.ufla.br/jspui/handle/1/57776
Appears in Collections:DMV - Artigos publicados em periódicos

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