Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/57830
Título: Cross-reactivity of commercially available anti-human monoclonal antibodies with canine cytokines: establishment of a reliable panel to detect the functional profile of peripheral blood lymphocytes by intracytoplasmic staining
Palavras-chave: Cytokines
Monoclonal antibodies
Cross-reactivity
Flow cytometry
Citocinas
Anticorpos monoclonais
Reatividade cruzada
Citometria de fluxo
Data do documento: 2015
Editor: BioMed Central
Citação: MOREIRA, M. L. et al. Cross-reactivity of commercially available anti-human monoclonal antibodies with canine cytokines: establishment of a reliable panel to detect the functional profile of peripheral blood lymphocytes by intracytoplasmic staining. Acta Veterinaria Scandinavica, Copenhagen, v. 57, n. 51, p. 1-12, 2015.
Resumo: The process for obtaining monoclonal antibodies against a specific antigen is very laborious, involves sophisticated technologies and it is not available in most research laboratories. Considering that most cytokines remain partially conserved among species during evolution, the search for antibody cross-reactivity is an important strategy for immunological studies in veterinary medicine. In this context, the amino acid sequence from human and canine cytokines have demonstrated 49–96 % homology, suggesting high probability of cross-reactivity amongst monoclonal antibodies. For this, 17 commercially available anti-human monoclonal antibodies [IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8 (#1, #2), IL-10, IL-12, IL-13, IL-17A, IFN-γ (#1, #2), TNF-α (#1, #2) and TGF-β], were evaluated in vitro for intracellular cytokine detection in a stimulated canine blood culture by flow cytometry and confocal microscopy. Lymphocytes from peripheral blood of healthy and two unhealthy dogs were analyzed.
URI: https://actavetscand.biomedcentral.com/articles/10.1186/s13028-015-0142-y
http://repositorio.ufla.br/jspui/handle/1/57830
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