Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/58851
Título: Protocol for in vitro rooting of Pyrus comunnis rootstocks
Palavras-chave: OHxF87 clone
PDW clone
Plant tissue culture
Pyrodwarf
Seedling production
6-Benzylaminopurine
Cultura de tecidos vegetais
Produção de mudas
Data do documento: 2023
Editor: Universidade Federal de Santa Maria
Citação: NADAL, M. C. et al. Protocol for in vitro rooting of Pyrus comunnis rootstocks. Ciência Rural, Santa Maria, v. 53, 2023.
Resumo: Effective protocols for in vitro rooting for woody fruit trees are still a challenge for in vitro seedling production, especially when there is a need to insert new cultivars or rootstocks. These protocols are essential to accelerate studies in plant breeding programs and for seedling distribution. This study evaluated the use of 6-Benzylaminopurine (IBA) in in vitro rooting of Pyrus comunnis rootstocks, clones ‘OHxF87’ and Pyrodwarf. Explant exposure times (0, 24, 48, 72, and 96 hours) to 20 mg L-1 IBA were tested for in vitro rooting. The exposure to IBA resulted in rooting rates above 80%, surpassing some results reported in the literature. The 24-hour treatment provided 81,81% survival, leading to an average growth of five roots with 19 mm length, for ‘OHxF87’ rootstock. The same exposure time resulted in the highest survival rate (75%) and the highest mean root number, seven roots per plant with 10 mm length, for ‘PDW’ rootstock. Root formation did not occur in the absence of synthetic auxin. Therefore, it can be concluded that a 24-hour exposure at 20 mg L-1 IBA was sufficient to promote in vitro rooting in ‘OHxF87’ and Pyrodwarf rootstocks’.
URI: http://repositorio.ufla.br/jspui/handle/1/58851
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