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Título: | Protocol for in vitro rooting of Pyrus comunnis rootstocks |
Palavras-chave: | OHxF87 clone PDW clone Plant tissue culture Pyrodwarf Seedling production 6-Benzylaminopurine Cultura de tecidos vegetais Produção de mudas |
Data do documento: | 2023 |
Editor: | Universidade Federal de Santa Maria |
Citação: | NADAL, M. C. et al. Protocol for in vitro rooting of Pyrus comunnis rootstocks. Ciência Rural, Santa Maria, v. 53, 2023. |
Resumo: | Effective protocols for in vitro rooting for woody fruit trees are still a challenge for in vitro seedling production, especially when there is a need to insert new cultivars or rootstocks. These protocols are essential to accelerate studies in plant breeding programs and for seedling distribution. This study evaluated the use of 6-Benzylaminopurine (IBA) in in vitro rooting of Pyrus comunnis rootstocks, clones ‘OHxF87’ and Pyrodwarf. Explant exposure times (0, 24, 48, 72, and 96 hours) to 20 mg L-1 IBA were tested for in vitro rooting. The exposure to IBA resulted in rooting rates above 80%, surpassing some results reported in the literature. The 24-hour treatment provided 81,81% survival, leading to an average growth of five roots with 19 mm length, for ‘OHxF87’ rootstock. The same exposure time resulted in the highest survival rate (75%) and the highest mean root number, seven roots per plant with 10 mm length, for ‘PDW’ rootstock. Root formation did not occur in the absence of synthetic auxin. Therefore, it can be concluded that a 24-hour exposure at 20 mg L-1 IBA was sufficient to promote in vitro rooting in ‘OHxF87’ and Pyrodwarf rootstocks’. |
URI: | http://repositorio.ufla.br/jspui/handle/1/58851 |
Aparece nas coleções: | DAG - Artigos publicados em periódicos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
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ARTIGO_Protocol for in vitro rooting of Pyrus comunnis rootstocks.pdf | 1,36 MB | Adobe PDF | Visualizar/Abrir |
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