Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/59269
Title: Prospecção dos efeitos de complexos vitamínicos hidrossolúveis, isolados e em associação a probióticos, na modulação de fosfolipases A2 e proteases
Other Titles: Prospection of the effects of water-soluble vitamin complexes, isolated and in association with probiotics, in the modulation of phospholipases A2 and proteases
Authors: Marcussi, Silvana
Preté, Paulo Sérgio Castilho
Marcussi, Silvana
Braga, Mariana Aparecida
Oliveira, Clayton Zambeli
Konig, Isaac Filipe Moreira
Pinto, Luciana de Matos Alves
Keywords: Suplementos alimentares
Lactobacillus
Inflamação
Hemostasia
Proteases
Fosfolipases A2
Dietary supplements
Inflammation
Hemostasis
Phospholipases A2
Issue Date: 26-Aug-2024
Publisher: Universidade Federal de Lavras
Citation: CARAPIÁ, M. S. Prospecção dos efeitos de complexos vitamínicos hidrossolúveis, isolados e em associação a probióticos, na modulação de fosfolipases A2 e proteases. 2024. 106 p. Tese (Doutorado em Agroquímica) - Universidade Federal de Lavras, Lavras, 2024.
Abstract: Vitamins are essential micronutrients for the normal metabolism of all living organisms and necessary in the regulation of vital biochemical reactions. Dietary supplements (vitamins, minerals and probiotics), used correctly and with specialized guidance, can improve health and well-being, resulting in increased quality of life, as well as ensuring ideal physiological functions. These bioactive compounds, vitamins and probiotic metabolites, are promising agents that modulate enzymes associated with inflammatory processes and hemostasis, such as proteases (e.g. serine proteases and metalloproteases), which act in processes such as digestion, blood coagulation and tissue regeneration, and phospholipases A2, involved in the inflammatory response, blood clotting and cell proliferation. In this study, vitamins B2 (30.85%) and vitamin B12 (25.53%) showed significant potential for inhibiting phospholipases A2. In silico analyzes indicate that these substances promote Van der Walls interactions in the catalytic site and complexation with the Ca2+ ion, forming stable complexes, resulting in a decrease in enzymatic activity. In vitro tests showed results of inhibition of hemolytic, coagulation and proteolytic activity in general, when the venom is subjected to treatments, mainly with vitamin C, B2 and B12. Associated with probiotic strains, the ability of vitamins C to inhibit the caseinolytic activity of Bothrops moojeni venom by 31.58% stands out. With the vitamin-probiotic association, it was possible to reduce 46.81% and 43.26% of hemolysis evaluated in solid media, using vitamin B2 and B12, respectively. The antioxidant activity of these substances and their complexation with metallic cofactors, such as Zn2+, are probably the mechanisms that justify the inhibitions observed in proteolytic assays. The results of the present study indicate that vitamin solutions, alone and in association with probiotic bacteria, have potential as an adjuvant in maintaining health, by mitigating the catalytic capacity of inflammatory enzymes. Thus, the bioactive substances investigated show promise for the development of new therapeutic and preventive strategies in the context of inflammatory diseases, as well as human health in general.
Description: Arquivo retido, a pedido do autor, até junho de 2025.
URI: http://repositorio.ufla.br/jspui/handle/1/59269
Appears in Collections:Agroquímica - Doutorado (Teses)

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