Identificação e avaliação do potencial celulolítico de fungos filamentosos isolados de área de canga da região do quadrilátero ferrífero

Abstract

The use of compounds produced by microorganisms moves a highly profitable market around the planet. Products of the metabolism of microorganisms gain relevance in several industrial segments, be it in the food, pharmaceutical, chemical, textile, among others. Among the microorganisms that produce biomolecules of interest and application, the filamentous fungi stand out, which in turn are good producers of enzymes. The objective of the present work was to evaluate the potential and quantify the enzymatic production of soil fungi from the mining area. For that, the fungi underwent a plaque evaluation, called qualitative. The result of the qualitative phase indicated that the largest producer of cellulases was the fungus Penicillium bilaiae. Therefore, the selected microorganism was subjected to submerged fermentation for the quantification of endoglucanases and β-glycosidases, with the inducing substrate being the flour of the bark of grapes. The peak activity of endoglucanases occurred in 120 hours of fermentation and with the production of 0.59 IU / mL, when the β-glycosidases were treated, the highest production was observed in the first 24 hours, with activity calculated at 1.77 IU / ml. Therefore, the selected isolate has potential application as a producer of enzymes, allowing for studies that involve the optimization of enzymatic production.