Inactivation of formyltransferase (wbkC) gene generates a Brucella abortus rough strain that is attenuated in macrophages and in mice

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dc.creatorLacerda, Thaís Lourdes Santos
dc.creatorCardoso, Patrícia Gomes
dc.creatorAlmeida, LeonardoAugusto de
dc.creatorCamargo, Ilana Lopes Baratella da Cunha
dc.creatorAfonso, Daniela Almeida Freitas
dc.creatorTrant, Cyntia Cardoso
dc.creatorMacedo, Gilson Costa
dc.creatorCampos, Eleonora
dc.creatorCravero, Silvio L.
dc.creatorSalcedo, Suzana P.
dc.creatorGorvel, Jean-Pierre
dc.creatorOliveira, Sérgio Costa
dc.date.accessioned2020-04-09T20:33:49Z
dc.date.available2020-04-09T20:33:49Z
dc.date.issued2010-08
dc.description.abstractRough mutants of Brucella abortus were generated by disruption of wbkC gene which encodes the formyltransferase enzyme involved in LPS biosynthesis. In bone marrow-derived macrophages the B. abortusΔwbkC mutants were attenuated, could not reach a replicative niche and induced higher levels of IL-12 and TNF-α when compared to parental smooth strains. Additionally, mutants exhibited attenuation in vivo in C57BL/6 and interferon regulatory factor-1 knockout mice. ΔwbkC mutant strains induced lower protective immunity in C56BL/6 than smooth vaccine S19 but similar to rough vaccine RB51. Finally, we demonstrated that Brucella wbkC is critical for LPS biosynthesis and full bacterial virulence.pt_BR
dc.identifier.citationLACERDA, T. L. S. et al. Inactivation of formyltransferase (wbkC) gene generates a Brucella abortus rough strain that is attenuated in macrophages and in mice. Vaccine, [S.l.], v. 28, n. 34, p. 5627-5634, Aug. 2010. DOI: 10.1016/j.vaccine.2010.06.023.pt_BR
dc.identifier.urihttps://repositorio.ufla.br/handle/1/39929
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0264410X10008236pt_BR
dc.languageen_USpt_BR
dc.publisherElsevierpt_BR
dc.rightsrestrictAccesspt_BR
dc.sourceVaccinept_BR
dc.subjectBrucella abortuspt_BR
dc.subjectRough strainpt_BR
dc.subjectLive vaccinept_BR
dc.titleInactivation of formyltransferase (wbkC) gene generates a Brucella abortus rough strain that is attenuated in macrophages and in micept_BR
dc.typeArtigopt_BR

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