Caracterização da resposta de Gliricidia sepium (Jacq.) steud. ao estresse salino mediante emprego da proteômica e metabolômica

Abstract

The world population is increasing rapidly over the years and it will be necessary to produce approximately 90% more food crops than we are producing today, especially grains, to meet this demand by 2050 (REDDY et al., 2017). However, abiotic stress, which includes salt, severely threatens agricultural production and causes significant yield losses in large areas (OSAKABE; OSAKABE, 2012). Thus, the general objective of the present study was applied to the analysis of the metabolome and proteome of leaves and roots of young plants of Gliricidia sepio, submitted or not to a high level of salt stress (> 25 dS m-1), before acquiring knowledge about the molecular mechanisms involved in this salinity tolerance. Therefore, data from the "Sal da Terra" database were used, belonging to the RD&I program of the same name developed at Embrapa Agroenergia, which houses data from physics, ionomics, genomics, transcriptomics (mRNA and microRNA), metabolomics and proteomics characterizing the response of oil palm (Elaeis guineensis), purslane (Portulaca oleracea) and gliricidia (Gliricidia sepium) to salt stress. How the metabolomes were analyzed on a UHPLC system equipped with a reversed phase column. High resolution mass spectrometry (HRMS) was performed on a Q-TOF analyzer using an electrospray source in ESI (+) - MS and ESI (-) - MS. The acquired data were pre-processed using XCMS Online and later exported to MetaboAnalyst for analysis, annotation and observation of metabolic pathways. Proteome Ame were prepared and submitted to LC - MS / MS analysis by the company GenOne (Rio de Janeiro, RJ, Brazil), using a "label-free" quantification approach, and the data were acquired using the Xcalibur software and subsequently exported to PatternLab for quantitative and qualitative analysis. The results achieved allowed to identify proteins and metabolites, and metabolic pathways, responsive to this stress, both in leaves and roots.