Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD
| dc.creator | Leal, C. A. G. | |
| dc.creator | Carvalho-Castro, G. A. | |
| dc.creator | Cottorello, A. C. | |
| dc.creator | Leite, R. C. | |
| dc.creator | Figueiredo, H. C. P. | |
| dc.date.accessioned | 2019-11-04T12:53:51Z | |
| dc.date.accessioned | 2023-06-27T19:57:30Z | |
| dc.date.available | 2019-11-04T12:53:51Z | |
| dc.date.available | 2023-06-27T19:57:30Z | |
| dc.date.issued | 2013 | |
| dc.description.abstract | The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples. | pt_BR |
| dc.identifier.citation | LEAL, C. A. G. et al. Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD. Brazilian Journal of Microbiology, São Paulo, v. 44, n. 3, July/Sept. 2013. | pt_BR |
| dc.identifier.uri | https://repositorio.ufla.br//handle/1/57882 | |
| dc.language | en_US | pt_BR |
| dc.publisher | Sociedade Brasileira de Microbiologia | pt_BR |
| dc.rights | Attribution-NonCommercial 4.0 International | * |
| dc.rights | Attribution-NonCommercial 4.0 International | |
| dc.rights | acesso aberto | pt_BR |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/ | * |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/ | |
| dc.source | Brazilian Journal of Microbiology | pt_BR |
| dc.subject | Clinical samples | pt_BR |
| dc.subject | Sensitivity | pt_BR |
| dc.subject | White spot syndrome virus (WSSV) | pt_BR |
| dc.subject | Real-time PCR | pt_BR |
| dc.subject | White spot disease (WSD) | pt_BR |
| dc.title | Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD | pt_BR |
| dc.type | Artigo | pt_BR |
