Avaliação da sensibilidade in vitro de isolados de Colletotrichum spp. à fungicidas

Abstract

Post-harvest diseases are among the main factors that reduce banana production. Among them the anthracnose stands out. Caused by the fungus Colletotrichum spp. this disease interferes in the quality of the fruits reducing their shelf life. As a control measure the use of chemicals has been the most used and with this has been observed the inefficiency of some active principles. Thus, the objective of this work was to evaluate the in vitro mycelial sensitivity of Colletotrichum spp. Isolates submitted to different doses of fungicides. The experimental design was completely randomized with 5 isolates, 3 fungicides, 4 doses (0.1, 1.0, 10, 100 mg L-1) and 3 replicates. Mycelial disks from each isolate were added on plates with the doses of each fungicide. Mycelial growth was measured after the fungal mycelium of the control plates reached their edges. The effective concentration of fungicides capable of inhibiting 50% of mycelial growth (EC 50) and classified into four efficiency categories was estimated. Statistical analyzes were processed using the SISVAR program. All the fungicides were efficient in the in vitro control, with emphasis on the microbiological. No resistance to any of the fungicides was observed for the isolates tested.