Protein recovery from barbados gooseberry (Pereskia aculeata Miller) leaves by salting out and isoelectric precipitation

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dc.creatorMorais, L. C.
dc.creatorNeves, I. C. O.
dc.creatorBatista, G. A.
dc.creatorSilva, M. L. M.
dc.creatorValentim, T. T.
dc.creatorMól, P. C. G.
dc.creatorResende, J. V.
dc.creatorThomasi, S. S.
dc.creatorVeríssimo, L. A. A.
dc.date.accessioned2020-04-15T17:47:47Z
dc.date.available2020-04-15T17:47:47Z
dc.date.issued2019
dc.description.abstractThis study evaluated the protein recovery from extract of Barbados gooseberry leaves by the synergistic effect of salting out and isoelectric precipitation using a factorial design, varying salt type ((NH4)2SO4, NaCl and KCl), salt concentration and temperature. Temperature elevation increased salting out effect. The optimum salt concentration was 0.5 mol/L for (NH4)2SO4 (66%), 1.5 mol/L for KCl (58%), and 2.5 for NaCl (62%). Research on isoelectric precipitation were performed. The most effective condition was found at 85 °C, using (NH4)2SO4 at 0.8 mol/L to 1.0 mol/L and pH = 1.0. Results showed the effectiveness of these effects on protein precipitation.pt_BR
dc.identifier.citationMORAIS, L. C. et al. Protein recovery from barbados gooseberry (Pereskia aculeata Miller) leaves by salting out and isoelectric precipitation. Revista Mexicana de Ingeniería Química, Ciudad de México, v. 18, n. 2, p. 419-430, 2019.pt_BR
dc.identifier.urihttps://repositorio.ufla.br/handle/1/40086
dc.languageen_USpt_BR
dc.publisherAcademia Mexicana de Investigación y Docencia en Ingeniería Químicapt_BR
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rightsacesso abertopt_BR
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceRevista Mexicana de Ingeniería Químicapt_BR
dc.subjectBarbados gooseberry - Mucilagept_BR
dc.subjectProtein concentratept_BR
dc.subjectSalting out extractionpt_BR
dc.subjectOra-pro-nobis - Mucilagempt_BR
dc.subjectConcentrado proteicopt_BR
dc.titleProtein recovery from barbados gooseberry (Pereskia aculeata Miller) leaves by salting out and isoelectric precipitationpt_BR
dc.typeArtigopt_BR

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