Desinfestação e meio de cultura para o estabelecimento in vitro de segmentos nodais de liquidambar styraciflua

Abstract

The objective of this research was to test the culture medium sterilization for the in vitroestablishment of Liquidambarstyraciflua L. nodal segments. Ministumps, from which the explants were collected, were propagated by cutting process and managed in clonal mini garden under semi-hydroponic system in a sand bed. The experiment was conducted in a completely randomized design under factorial arrangement (3x2x2); the factors were: three clones (L 26, L 35 and L 63), two sterilization methods (A1 - sodium hypochlorite (2.5% v/v of active chlorine) during 10 minutes; A2 - explants immersed during 40 minutes in a solution of benomyl 1% w/v) and two culture mediums (WPM and MS), with four replications. The clones did not differ in relation to the asepsis and culturemedium. In average, 4% of the explants were free of contamination. However, almost 80% of the material presented bacterial contamination, what indicated the necessity of developing a more efficient sterilization protocol. Although only a little difference between the culture mediums had been observed, the MS medium showed superiority in relation to the WPM regarding the majority of the morphological characteristics studied.