Determinação de anestésicos residuais em filés de cachadia (Pseudoplatystoma reticulatum x Leiarius marmoratus) e tilápia (Oreochromis niloticus)

Abstract

In this work the residual quantification of the anesthetics clove oil, benzocaine and tricaine was performed for fillets of two species of fish, one leather species, “cachadia” (Pseudoplatystoma reticulatum x Leiarius marmoratus), and other scale species, Nile tilapia (Oreochromis niloticus) through solid-liquid extraction (SLE) and in solid phase (SPE), followed by analysis in high performance liquid chromatography with diode arrays detector (HPLC – DAD). Anesthesia was evaluated in five different contents defined according to the induction time for each species and four depuration times (0 h, 12 h, 24 h and 48 h) in four repetitions. The variance of residual means among anesthetics, contents and fish species was compared. After anesthesia (0 h), the species presented residues of benzocaine corresponding to 1.46 μg g-1 and 2.6 μg g-1, tricaine 0.397 μg g-1 and 2.17 μg g-1 and eugenol 0.514 μg g-1 and 0.987 μg g-1, respectively. Fishes depurated during 12 h, 24 h and 48 h presented values below detection and quantification limits. Benzocaine presented the highest residual mean contents for tilapia and “cachadia” (p<0.01) in function of its low solubility. Tricaine presented lower residual means for tilapia and eugenol for “cachadia”. There was no difference among the five applied contents (p>0.01), except for the lowest content of tricaine in tilapia, which resulted in higher residual contents. Lastly, mean values of residues in “cachadia” were higher than tilapia. Thereby, regarding the residual analysis, tricaine and eugenol are the most indicated anesthetics for tilapia and “cachadia”, respectively