Mesos components (CaCl2, MgSO4, KH2PO4) induced changes in growth and ascaridole content of Dysphania ambrosioides L. in vitro

Abstract

The mesos components are the nutrients required by intermediate concentrations and recently, it are identified as one of the most influential groups of nutrients in Murashige and Skoog medium. Dysphania ambrosioides L. is a medicinal species used mainly as anti-helminthic around the world. The aim of the present study is to assess this species’ growth and volatile constituent parameters in vitro based on mineral nutrition with different components concentrations. Different magnesium sulfate – MgSO4 (0; 185; 370; 740; 1480 mg L−1), calcium chloride – CaCl2 (0; 220; 440; 880; 1760 mg L−1), and monopotassium phosphate – KH2PO4 (0; 85; 170; 340; 680 mg L−1) concentrations in the Murashige and Skoog medium were tested in the nodal segments. Overall, components influenced the growth of Dysphania ambrosioides L. nodal segments in vitro. The most adequate components intervals on MS medium for the maintenance of Dysphania ambrosioides L. nodal segments in vitro are: from 1039 to 1069 mg L−1 of MgSO4, from 958 to 1301 mg L−1 of CaCl2, and from 329 to 397 mg L−1 of KH2PO4. The content in main volatile fraction constituents was influenced by different components concentrations in vitro. For treatments with 0 and 1480 mg L−1 of MgSO4 and 0 mg L−1 CaCl2 had more ascaridole content. The analysis of loadings allowed observing that ascaridole had negative correlation with cymene.