Neutrophil-generated HOCL leads to non-specific thiol oxidation in phagocytized bacteria

dc.creatorDegrossoli, Adriana
dc.creatorMüller, Alexandra
dc.creatorXie, Kaibo
dc.creatorSchneider, Jannis F.
dc.creatorBader, Verian
dc.creatorWinklhofer, Konstanze F.
dc.creatorMeyer, Andreas J.
dc.creatorLeichert, Lars I.
dc.date.accessioned2019-02-19T17:06:56Z
dc.date.available2019-02-19T17:06:56Z
dc.date.issued2018
dc.description.abstractPhagocytic immune cells kill pathogens in the phagolysosomal compartment with a cocktail of antimicrobial agents. Chief among them are reactive species produced in the so-called oxidative burst. Here, we show that bacteria exposed to a neutrophil-like cell line experience a rapid and massive oxidation of cytosolic thiols. Using roGFP2-based fusion probes, we could show that this massive breakdown of the thiol redox homeostasis was dependent on phagocytosis, presence of NADPH oxidase and ultimately myeloperoxidase. Interestingly, the redox-mediated fluorescence change in bacteria expressing a glutathione-specific Grx1-roGFP2 fusion protein or an unfused roGFP2 showed highly similar reaction kinetics to the ones observed with roGFP2-Orp1, under all conditions tested. We recently observed such an indiscriminate oxidation of roGFP2-based fusion probes by HOCl with fast kinetics in vitro. In line with these observations, abating HOCl production in immune cells with a myeloperoxidase inhibitor significantly attenuated the oxidation of all three probes in bacteria.pt_BR
dc.identifier.citationDEGROSSOLI, A. et al. Neutrophil-generated HOCL leads to non-specific thiol oxidation in phagocytized bacteria. Elife, [S.l.], 2018.pt_BR
dc.identifier.urihttps://repositorio.ufla.br/handle/1/32848
dc.identifier.urihttps://elifesciences.org/articles/32288pt_BR
dc.languageen_USpt_BR
dc.rightsopenAccesspt_BR
dc.sourceeLifept_BR
dc.titleNeutrophil-generated HOCL leads to non-specific thiol oxidation in phagocytized bacteriapt_BR
dc.typeArtigopt_BR

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