Estudo da compatibilidade entre clones de Coffea canephora por meio de marcadores SNPs

Abstract

Conilon coffee belongs to the Coffea canephora species, which is a diploid (2n = 2 × = 22), perennial, allogamous plant with a high genetic variability. Accordingly, the production of clonal varieties is of great value for the success of crop. Coffee is one of the most important agricultural commodities in the world. Brazil is the largest producer, responding by 30% of the world production. C. canephora comprises 37% of the world production and 24% of the Brazilian coffee production. C. canephora has gametophytic self-incompatibility, when haploid pollen matches one of the same allele present in the diploid style causing pollen rejection. Therefore, the objective of this work was to study the compatibility between 13 clones of C. canephora, denominated CCEs, by SNPs markers. Our methodology was efficient to reconstruct the pedigree of 10 CCEs progenies, identifying correctly the maternal parent in most analyzed plants for all CCEs, with the use of SNPs markers. Results have also shown a genotypic similarity of 99.8% between CCE04 and CCE11 clones, suggesting that these plants may be identical. Parental analyzes performed with the Cervus Software proved to be efficient to inferring the compatibility between all tested clones, and the CCE07 clone was compatible with all other CCEs tested, which could indicate a high productive potential for this clone, which was experimentally confirmed.