Qualidade de gametas com a técnica de endoscopia em curimba (Prochilodus lineatus)

Abstract

Endoscopy can facilitate for fish and allows the performance of sexed accurately, even if its use is still restricted. The curimba is a rheophilic species of fish, used in experiments as a model species for migratory fish. This study aimed to enable the use of the endoscope in sexing breeding curimba and determine whether the technique alters the reproductive rates. Curimbas 16 were examined, in which sex was identified by the introduction of the pore urogenital endoscope for viewing the gonads. The endoscope used (model Multi-Purpose Rigid ™, Karl Storz, Germany) had a 30° angle with 2.7 mm diameter and 18 cm in length. Hormonal induction was performed 20 days after application of the technique, the females received two doses of crude extract of carp pituitary (CECP) of 0.4 and 4.0 mg / kg of fish, with an interval of 12 hours and the males receiving a single dose of 4.0 mg / kg of fish CECP, when the second application in females. After 12 hours, we proceeded to the extrusion of the gametes, which was removed a semen sample for evaluation of morphology, rate (%) and duration (seconds) motility and sperm concentration. For the females took place evaluating the weight of spawning and collecting a sample of oocytes to evaluate the position of the germinal vesicle and oocyte diameter. It can be observed that 100% of males and 62% of female spawned. In addition, all reproductive indices were considered within the standards expected for the species. It follows that the endoscope can be used efficiently in sexing and evaluating the degree of maturation prior to spawning season, without changing the reproductive efficiency of Fish.