Ação do BAP na regeneração in vitro de Blc Owen Holmes Ponkan x Brassavola digbiana nº 2

Abstract

Cattleya is considered the most beautiful and searched among all orchids. This fact creates the need of developing more effective propagation techniques to become available a greater number of individuals in reduced periods of time and physical space, besides maintenance of uniformity and genotipic identity to meet the needs of the market. The objective of this work was to evaluate the regeneration of the cultivated shoot meristematic in vitro of the hybrid Blc Owen Holmes Ponkan x Brassavola digbiana nº 2 in the different BAP (6-benzylaminopurine) concentrations. The explants were inoculated in the liquid medium with or without growth regulators in the following combinations: T1: MS liquid + coconut water; T2: MS + coconut water + 0.1 mg L-1 NAA + 0.2 mg L-1 BAP; T3: MS + coconut water + 0.1 mg L-1 NAA + 0.5 mg L-1 BAP and T4: MS + coconut water + 0.1 mg L-1 NAA + 1.0 mg L-1 BAP. Subcultivatings and evaluations (percentage of survival, contamination, medium color, oxidation and regeneration) were made at 18, 32, 62, 93, 123, 153, and 184 days. At 93 days the first signs of regeneration were observed. BAP (1.0 mg L-1) enhanced good results with the formation of 132 sprouts in the 184 days, concluded that the utilization of BAP improved morphogenic response with a higher number of shoots.