Avaliação do fungo Duddingtonia flagrans e hipoclorito de sódio a 5% sobre a eclodibilidade de ovos de ciatostomíneos

Abstract

The aim of this study was to assess various concentrations of conidia from the fungus Duddingtonia flagrans (AC001) and sodium hypochlorite (NaOCl) at a 5% (v/v) solution in relation to the hatching of equine gastrointestinal nematode eggs. Five experimental groups were established: Group 1 (3 x 106 conidia of AC001 + 20 g of feces), Group 2 (6 x 106 conidia of AC001 + 20 g of feces), Group 3 (9 x 106 conidia of AC001 + 20 g of feces), Group 4 (5 mL of sodium hypochlorite at 5% (v/v) + 20 g of feces), and Group 5 (5 mL of distilled water + 20 g of feces). The coprocultures were then incubated for 10 days in the dark at a temperature of 26 ± 1º to 2ºC. Subsequently, infective larvae (L3) of nematodes were recovered using the Baermann technique. At the end of the experimental period, the absolute presence of cyathostomin nematodes’ L3 was confirmed. The results demonstrated a significant decrease (p<0.01) in L3 recovery in all groups treated with AC001 (G1, G2, and G3) as well as with 5% (v/v) sodium hypochlorite (G4) compared to the control group (G5). No significant difference (p>0.01) was observed between the three concentrations of conidia tested in groups G2 and G3. In conclusion, both the fungus D. flagrans and 5% (v/v) sodium hypochlorite effectively reduced L3 counts in stool cultures, suggesting their potential use in future experimental designs.