Meio de cultura e luz na micropropagação do abacaxizeiro Gomo de Mel

Abstract

The pineapple, belonging to the family of bromeliads, is native of South America and cultivated in any hot region. It has wide acceptance throughout the world, both natural and industrialized. Brazil is the 2nd largest producer of fruit, producing several varieties to meet both the internal and external. The Agronomy Institute of Campinas - IAC launched in 1999, a variety of pineapple from China that became known as 'Gomo de mel', from natural breeding, showing very interesting features for the consumer. Due to limited information on the variety and the low availability of seedlings for the producer, this work aimed at using the technique of plant tissue culture, get a higher production of seedlings of the pineapple 'Gomo de mel' by testing different doses of growth regulators, sucrose, light quality and physical composition of the medium. Three experiments were conducted: 1) production of seedlings by the method of shading in different concentrations of BAP (0, 0.25, 0.5 and 1.0 mg.L-1) and GA3 (0, 0.5, 1, 0 and 2.0 mg.L-1), 2) quality of light (white, red, blue) and concentration of sucrose (0, 10.0, 20.0, 30.0 g) and 3) the constitution medium (solid, liquid, liquid with agitation) and concentration of BAP (0, 0.5, 1.0, 1.5, 2.0 mg.L-1). The best results were obtained with the addition of 1.0 - 1.5 mg L-1 BAP, with the greatest achievement of brotos.Para better shoot growth is recommended 2.0 mg L-1 GA3 the maximum sucrose, 30g L-1, added to the medium allows a better development of the explant, as well as in vitro growth of the plant under white light. Micropropagation of pineapple in liquid medium with constant agitation produces healthy, vigorous seedlings for the acclimatization phase.