Cultivo de cardiomiócitos suínos

Abstract

Cardiac insufficiency is one of the most harmful disease in the occidental world; A range of researches have been developed in order to provide new and more efficient therapeutics for the disease. New therapeutics purposes have been directed to the origin of the pathological process and aim to re-establish functional physiology, as in transplants of cells like smooth or striated muscular cells, fetal or adult cardiomyocytes into the fibroid myocardium. In this study, previous published protocols developed in rats and mice were adapted for swine cardiomyocytes. The main goal was to stablish a viable protocol for swine cardiomiocyte culture, and the establishment of an experimental model using swine. Three protocols were tested (A - enzimatic digestion in adult heart; B- enzimatic digestion in fetal heart; C - explant of samples of adult and fetal hearts. We observed that protocol Bproduced a reliable result suggesting cardiac fibre formation, although, further immunecitochemistry and ultrastrucutural will be investigated to confirm the hypothesis.