Efeito de bactérias endofíticas no controle de Meloidogyne incognita e sua capacidade de colonização de raízes de tomateiro

Abstract

This work aimed to evaluate the effect of bacteria on the reproduction of Meloidogyne incognita and their capacity to colonize the tomato root using the Phytagel® technique. Tomato seeds were embedded into bacterial suspension OD540 = 0.5 for 24 hours. Then, the inoculated seeds were sown in 300 cm3 cups filled with Plantmax® substract. Fifteen days later, seedlings were inoculated with 400 eggs of M. incognita. Thirty days later, the numbers of galls, egg-masses and eggs per gram of roots were evaluated. For the colonization assay, the tomato seeds were embedded in bacterial suspension and then placed to germinate in sterile tubes with Phytagel®. The tubes were kept in growth chamber without light, at 28 °C. The tubes were checked daily for bacterial growth. The species that gave better control of M. incognita were: Acinetobacter johnsonii (T9 e T10), Curtobacterium luteum (P16), Bacillus pumilus subg. B (T27), B. pumilus (T26) e B. amyloliquefaciens (T36), which also colonized the tomato roots.