Aspectos do cultivo in vitro do manjericão cv. maria bonita (Ocimum basilicum L.)

Abstract

Two experiments were carried out with the objective to evaluate the influence of growth regulators on in vitro multiplication of sweet basil (Ocimum basilicum L.) cv. Maria Bonita. The first experiment had the objective to verify the effect of different concentrations (0.0; 0.5 and 1.0 mg L -1) of BAP and NAA on sweet basil plantlets formation and the second one was to evaluate callus induced by using different concentrations of 2,4-D (0.0; 0.5; 1.0 and 2.0 mg L -1) on leaf explants. The experiments used a randomized complete design with 9 and 4 treatments in the first and second experiments, respectively. In the first experiment, shoot length, dry and fresh biomass and dry and fresh callus biomass were evaluated. It was observed that shoot length, fresh and dry shoot biomass were better without the growth regulators. In the second experiment, only dry and fresh biomasses were evaluated. It observed that shoot length and fresh and dry biomasses were superior in the absence of growth regulators. Regarding the presence of callus in nodal segment, the best used concentration was the combination of 0.5 mg L-1 NAA and 0.5mg L-1 BAP. Higher leaf callus fresh biomass (2.10g) was obtained in MS supplemented 1.26 mg L -1 2,4-D. Higher leaf dry callus biomass (1.18g) was obtained with 0.31 mg L-1. With the increase of 2,4-D concentration, it was observed a decrease in both fresh and dry biomass.